@ Max Delbrück Center for Molecular Medicine, Germany
Regulatory RNAs & Single Cell Sequencing
In the first part of my talk I will discuss circular RNAs (circRNAs). circRNAs have recently emerged as an interesting, large class of animal RNAs with mostly unknown function. I will present loss-of-function data in mouse brain independently for two circular RNAs and describe our understanding of the molecular and functional consequences.
In the second part of my talk I will present projects where we employ massively parallel, high-throughput single-cell RNA sequencing. I will present (a) the perhaps first “complete” cell/lineage atlas of a complex animal (b) (collaboration with Zinzen lab) a “virtual embryo” where we have sequenced each cell of the drosophila embryo at stage 6 and reconstructed gene expression for 8,000 genes per cell in 3-D, (c) data about single-cell sequencing of an animal germline which reveal spatial organization of functional classes of RNA.